Protein_Domain

Part:BBa_K5466008:Design

Designed by: Adrián Gómez Lara, Daniel Bulnes Roldán   Group: iGEM24_UMA-MALAGA   (2024-09-23)


NubG


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Spontaneous reconstitution of the split protein is prevented through mutation I13G and a flexible linker 2x (GGGGS) has been added.

S. cerevisiae codon optimized.


Source

Nub domain of iGEM20_UCopenhagen BBa_K3617000.